GENFINE BIOTECH (BEIJING) CO., LTD

[New product] Flash Robust hot start DNA polymerase, something you don't know

At present, PCR technology can be said to be developing rapidly and becoming more mature and widespread. However, various problems still arise in PCR experiments, such as high Ct or no value, or the amplification efficiency always not reaching the expected 90% -110%. This may be due to unreasonable primer design or the need to optimize the reaction system, but it may also be due to sample inhibitors affecting PCR amplification. There are two common types of inhibitors:
1) Endogenous components of the sample itself, such as hemoglobin, immunoglobulin G, Lactoferrin, etc.
2) Exogenous, extract components from reagents or consumables, such as phenol, chloroform, ETDA, heparin, or citrate.
In PCR reactions, inhibitors can directly bind to Taq enzymes, affecting their active groups, and many cofactors of Taq enzymes may also be the target of PCR inhibitors. For example, many PCR inhibitors can bind to Mg2+, thereby reducing Mg2+activity or hindering its binding to polymerase and inhibiting PCR reactions. In order to solve the interference problem of inhibitors in PCR experiments, Jifan has launched a super resistant enzyme that can be compatible with multiple complex templates.
 

超闪抗逆热启动DNA聚合酶

Flash Robust HotStart DNA Polymerase

Product Introduction:
This product is a new type of hot start polymerase derived from wild-type Taq Pol through targeted modification, which has strong resistance to blood and PCR inhibitors. Adopting dual antibody blocking, it has ultra-high amplification efficiency, good amplification sensitivity, and specificity. The product is heated at the pre denaturation temperature for 30 seconds, and the blocking antibody can completely inactivate, releasing DNA polymerase activity.
Product features:
Ultra fast amplification: The amplification speed of fragments within 3kb of complex template amplification can reach 5-10 seconds/kb, and the amplification speed of simple template amplification can reach 1 second/kb.
Super stress resistance: It can tolerate a variety of high concentration inhibitors, such as EDTA, Guanidinium chloride, heparin, Sodium acetate, etc.
Ultra high sensitivity: PCR&qPCR can achieve effective amplification of individual copy numbers.
Direct expansion of blood: can effectively expand 20% of blood volume (qPCR method).
Performance demonstration:
It is resistant to a variety of high concentration inhibitors, such as EDTA, Guanidinium chloride, heparin, Sodium acetate, etc. It can be used for direct expansion of blood and can effectively expand a 20% proportion of whole blood samples.

Impurity tolerance test

A210 and the competing products were tested for impurity tolerance respectively. Under the interference of EDTA, Guanidinium chloride and NaCl of the same concentration, A210 sensitivity and fluorescence value were better than the competing products; Under the same concentration of SDS interference, there is no significant difference in performance between A210 and its competitors.
 

Blood direct expansion comparative experiment
Using non plague positive blood for direct blood expansion, A210 can amplify 20% of the blood sample, while the competitor has no amplification. The results are as follows:
 
 

Direct expansion experiments with different plasma ratios
In the plasma direct expansion qPCR system, A210 can amplify 30% of plasma samples.
 

After reading the introduction of Flash Robust HotStart DNA Polymerase (A210), will you still worry about the impact of PCR inhibitors on the experiment? Choosing A210 simplifies your experiment, eliminates the troubles of PCR inhibitors, and directly performs 20% blood expansion. A210 helps you solve this problem. We are ready! Welcome to inquire and order!
Ordering Information:

 

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